Anne Carter Lee died of typhoid fever at Jones Springs, North Carolina, on October 20, 1862, aged twenty-three. Her illness began in August during the family's wartime displacement from Arlington. She and her sister Agnes went farther south hoping for improvement, but headaches, dizziness, and intestinal difficulties became worse. At first recovery still seemed possible. As the illness deepened, their mother Mary came from Virginia, and mother and sister shared the nursing. The National Park Service account describes Agnes caring for Annie by day and Mary at night. Annie eventually became unconscious; Agnes tried to keep her warm, and Mary was holding her hands when she died on the morning of October 20. These details describe a decline over weeks rather than an abrupt battlefield or travel death. Robert E. Lee received the news by letter and was absent from the bedside. The Jones family offered a place in its private cemetery near Warrenton, where Annie was buried. Her remains were moved to Lexington in 1994. The known symptoms and family care support a specific account of the fatal illness, while the exact medications and other medical measures remain undescribed.